A study on pgd2 and androgens relationship

[StayPositive]

Prostaglandin D2-Mediated DP2 and AKT Signal Regulate the Activation of Androgen Receptors in Human Dermal Papilla Cells

Prostaglandin D2 (PGD2) and prostaglandin D2 receptor 2 (DP2) is known to be an important factor in androgenetic alopecia (Androgenetic Alopecia). However, the effect of PGD2 in human dermal papilla cells (hDPCs) is not fully understood. The function of PGD2-induced expression ...

www.ncbi.nlm.nih.gov

5. Conclusions
PGD2 directly stimulates the expression of androgen target genes, AKT and its downstream substrates are involved in mediating these effects. Thus, our data in this study provide that the activity of AR could be regulated not only DHT but also various signal changes by PGD2 in hDPCs.

 

[StayPositive]

We next examined whether a DP2 antagonist affects AR expression. hDPCs were pretreated with TM30089 (DP2 antagonist) at 20 μM for 1 h and then treated with 100 nM DHT for 5 h. Stimulation with TM30089 inhibited the upregulation of AR and DP2 (Figure 1F,G). Additionally, immunocytochemistry data showed that DHT-stimulated AR and DP2 expression was detected in the nucleus, and the expression of AR and DP2 in the TM30089-treated group was weaker than that in the DHT-treated group (Figure S1A,B).

 

[StayPositive]

Prostaglandin D2 receptor (DP2) antagonist (TM30089) decreases dihydrotestosterone (DHT)-induced androgen receptor (AR) and prostaglandin expression in human dermal papilla cells (hDPCs). The mRNA expression of cyclooxygenase-2 (COX2), prostaglandin D2 synthase (PTGDS) and DP2 was examined in hDPCs treated with DHT for 24 h. The mRNA expression of COX2 (A), PTGDS (B) and DP2 (C) was induced by 100 nM DHT. DP2 protein expression was strongly induced by 100 nM DHT at 5 h (D). hDPCs were cultured for 24 h with DHT, as indicated. The level of PGD2 receptor in the supernatant was evaluated in three independent experiments (E). The relative mRNA levels were normalized to that of GAPDH. hDPCs were pretreated with 20 µM TM30089 for 1 h and then treated with 100 nM DHT for 5 h. The protein level of AR (F) and DP2 (G) was measured by western blot. TM30089 decreased the DHT-induced AR and DP2 expression. β-actin served as a loading control for protein normalization. The results are expressed as the mean  ±  SD of three independent experiments: CTL; control. * p < 0.05 compared with the control (0 nM DHT). # p < 0.05 compared with the DHT 100 nM.

 

[StayPositive]

I am convinced PGD2 is the key guys

 

[StayPositive]

This is huge:

Prostaglandin D2 receptor (DP2) antagonist (TM30089) decreases dihydrotestosterone (DHT)-induced androgen receptor (AR) and prostaglandin expression in human dermal papilla cells (hDPCs)

 

[killDHT]

wow，you are so good

 

[StayPositive]

wow，you are so good

What's your experience on diclofenac + castor oil combo?

 

[Charger]

Putting in an order for TM30089 today, gonna need to find a place to get it tested before I take it orally.

 

[StayPositive]

Putting in an order for TM30089 today, gonna need to find a place to get it tested before I take it orally.

Be careful, TM is very powerful, caused sleep problems in every guys who tried it in the past. It lowers pgd2 activity too much, messing with sleep.

 

[Charger]

Be careful, TM is very powerful, caused sleep problems in every guys who tried it in the past. It lowers pgd2 activity too much, messing with sleep.

Seems some have been able to offset that with melatonin or other supplements.

Obviously, won't know my tolerability until I try it, but I'll take that side effect over anything that messes with testosterone/dht or 5ar, personally.

 

[Charger]

Personally, I just want to replicate westonci's results. And while he said that he felt PGE2+Microneedling were the heavy hitters, the inhibition of PGD2 more than likely allows growth agonists such as PGE2, microneedling, etc. to be that much more effective.

I think for any progress to be made, it's going to comedown to inhibition of androgens or the prostaglandins they upregulate. I know the answer for me isn't inhibiting DHT because I simply can't tolerate that route, so it's either this route or nothing.

Want to see what TM+PGE2+PGF2a does for me, and maybe eventually add SW033291 as pegasus seems pretty big on it.

 

[StayPositive]

What's your opinion about setipiprant failing trial for hairloss?

And sucks fevi was also not good enough against astma.

Seti is just too weak in my opinion, and so f*****g pricey. Cetirizine would be a better alternative for pgd2, it's cheap and reduces pgd2 by around 50%

If your going for a full prostaglandins protocol like myself, i suggest high dose asprin / oral castor oil / niacin combo

I am currently trying this protocol

 

[StayPositive]

Personally, I just want to replicate westonci's results. And while he said that he felt PGE2+Microneedling were the heavy hitters, the inhibition of PGD2 more than likely allows growth agonists such as PGE2, microneedling, etc. to be that much more effective.

I think for any progress to be made, it's going to comedown to inhibition of androgens or the prostaglandins they upregulate. I know the answer for me isn't inhibiting DHT because I simply can't tolerate that route, so it's either this route or nothing.

Want to see what TM+PGE2+PGF2a does for me, and maybe eventually add SW033291 as pegasus seems pretty big on it.

He tried the prostaglandins protocol with the worst pgd2 inhibitor we have.

Yes i think keeping pge2 activity high is very important too, but if pgd2 activity is not reduced, you will continue to lose your hair over time. This is what the guys who only use minoxidil experience. They increase pge2 without decreasing pgd2 which is attacking the follicules.

Microneedling is very powerful, but sadly i have Diffuse thinning all over my scalp (DUPA), so it's impossible for me to entirely cover it

 

[Lurker otto]

Seti is just too weak in my opinion, and so f*****g pricey. Cetirizine would be a better alternative for pgd2, it's cheap and reduces pgd2 by around 50%

If your going for a full prostaglandins protocol like myself, i suggest high dose asprin / oral castor oil / niacin combo

I am currently trying this protocol

Do you know how much pgd2 is reduced by niacin?
So in theory if i add niacin to my regimen may be things like minoxidil can act better?

 

[Isneezedsohard]

Prostaglandin D2-Mediated DP2 and AKT Signal Regulate the Activation of Androgen Receptors in Human Dermal Papilla Cells

Prostaglandin D2 (PGD2) and prostaglandin D2 receptor 2 (DP2) is known to be an important factor in androgenetic alopecia (Androgenetic Alopecia). However, the effect of PGD2 in human dermal papilla cells (hDPCs) is not fully understood. The function of PGD2-induced expression ...

www.ncbi.nlm.nih.gov

5. Conclusions
PGD2 directly stimulates the expression of androgen target genes, AKT and its downstream substrates are involved in mediating these effects. Thus, our data in this study provide that the activity of AR could be regulated not only DHT but also various signal changes by PGD2 in hDPCs.

This is huge

 

[Isneezedsohard]

Do you know how much pgd2 is reduced by niacin?
So in theory if i add niacin to my regimen may be things like minoxidil can act better?

I thought I was imagining it— but my shedding has reduced 10 fold since incorporating niacin flushes every other day

 

[Isneezedsohard]

I am using cetirzine before i was balding . And here i am here . Also tried it topical version which i made based on the research post and got 0 result. I think the pgd2 path was over many tried it never deliver anyt

I am using cetirzine before i was balding . And here i am here . Also tried it topical version which i made based on the research post and got 0 result. I think the pgd2 path was over many tried it never deliver anything.

I think part of it is finding what works for you. Same thing with finasteride, dutasteride, etc. sometimes you need more. I’m doing castor+niacin. Which seems to work for me

 

[Isneezedsohard]

You lost me on castor oil...

Oral castor oil inhibits pgd2 (bad for hair) and promotes pge2 (good for hair)

 

[Isneezedsohard]

You talk about like its no the first choice of ppl that dont want to use finasteride. We been there . Dosent work. We have like 400 studys of things that work on paper but not in real life. That the bitter truth

Not true at all. First off— I never said “it’s the first choice of ppl”. That’s categorically untrue, and I never claimed that. Second we have tons of studies and anecdotal evidence that PGE2 and PGD2 play some role in male pattern baldness (among countless other factors). We are all trying to figure out how to crack the balding “code”, but it’s a FACT that they’ve noticed a correlation between Prostaglandins and balding. At the end of the day, I don’t believe it’s the main cause (I believe androgens are, most likely) but it does have a role in the cascade.

 

[Charger]

lol @ roeysdomi, I remember that nutter from tressless.

Anyway, do you guys not notice any negatives from niacin flushes? No effects on your mood or anything?