docj077
Senior Member
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S Foote. said:
That isn't MY claim and I grow tired of your "scientific" observations and opinions. That is the claim of pretty much everyone that studies hair loss except for you...and Armando.
I keep forgetting that you have a Ph.D. in molecular biology <sarcasm>.
You seem to ignore the very opinion of the people that study this phenomenon on a daily basis.
Good for you, but bad for the forum.
http://www.nature.com/jidsp/journal/v8/ ... .html#fig2
"Secretion of TGF-1 into the medium from cocultured of KCs and DPCs from bald patients
TGF-1 is known to inhibit human KC growth in vitro (Shipley et al, 1986), and the expression level of its receptor in epithelial cells of hair follicles is tightly regulated during the hair cycle (Paus et al, 1997). Moreover, TGF-1 -/– mice exhibit delayed catagen progression (Foitzik et al, 2000) and exogenous administration of TGF-1 hair follicles in vitro to induces catagen progression (Soma et al, 1998). Therefore, we focused on TGF-1 as a candidate mediator in the androgen-induced growth suppression of KCs. To examine whether TGF-1 production is up-regulated by androgen, we measured the concentration of TGF-1 in the conditioned medium after the coculture of KCs and bald DPCs for four d using an ELISA assay. The concentration of TGF-1 was 69.0 or 184.7 pg/ml in the conditioned medium treated with ethanol or 10-9M R1881, respectively (Figure 3), showing that androgen stimulates the production of TGF-1 in this coculture."
"Reversal by TGF-1 neutralization of androgen-induced growth inhibition of KCs
To confirm the function of TGF-1 in androgen-induced growth inhibition of KCs, we examined the effect of the neutralizing antibody against human TGF-1 on this growth suppression in cocultures of KCS and bald DPCs. Neutralizing anti-TGF-1 almost completely reversed the inhibition of proliferation of KCs by 10-9M R1881 in this coculture system (Figure 4), demonstrating that TGF-1 mediates the signal from DPCs to KCS during growth suppression."
So, now what are you talking about with regards to TGF-beta? Do you even know? Do you have a study that involves injecting TGF-beta into human scalp follicles in-vivo and it didn't demonstrate inhibition, because I'd like to read such an article?
To ignore the above, seems very foolish on your part. As you can see, TGF-beta null mice demonstrate delayed catagen progression. Not only is this true, but androgens have been clearly found to upregulate TGF-beta in culture. Another study has been posted on this forum that clearly demonstrates that TGF-beta is increased in-vivo, as well. Lastly, antibody against TGF-beta reverses the inhibitory effects of TGF-beta produced by the dermal papillae cells and restores normal keratinocyte function.
And, by the way, the following is the opinion and the observation of the individuals performing this study:
"In cocultures, R1881 had no significant effect on the proliferation of DPCs (data not shown). These results suggested that this in vitro coculture of DPCs and KCs reproduces very well the in vivo interaction between DPCs and KCs, especially in terms of androgen effects on hair growth."
You can argue all you want, but the actual people with the credentials to have an opinion say that what they've done "reproduces very well the in vivo interaction between DPCs and KCs, especially in terms of androgen effects on hair growth." So, I have to ask, what makes you more qualified than these individuals when you say that these studies aren't sufficient or that these growing conditions aren't correct or when you have some other opinion that goes against the knowledge and the opinion of your scientific superior? I have to ask, because just as you think that I'm bad for the forums, I'm starting to think that you're far worse. To openly proclaim theories that directly contradict the opinion of hair loss researchers with years of experience and then you tell people that the answer is edema and that cooling of the scalp will help the condition. I'm surprised you haven't been removed from this forum. At least Bryan, Michael, and few others study the articles and draw conclusion based upon the conclusions of the authors. What you're doing is almost an insult to the intelligensia running these experiments.
So, you can think what you want, but NOBODY else in the world of hair loss thinks the same as you.
All the studies that people keep posting around here dealing with TGF-beta and androgen mediated inhibition of hair growth are three years older or more. Where have you been that you're unable to conform to the scientific norm? Did you wake up one day and decide that coming up with a theory with no biochemical or physiological basis behind it was the answer to sustaining your life? You don't have the histology to back up your claims, nor do you have the pathophysiology.
And, by the way, was coming on here and claiming that you have a life outside of this forum just some attempt to establish some sort of sense of self-importance? You have a theory with a website for that theory. Who is the one with a life outside of the forums, again?
