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Composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin c derivatives
USPTO Application #: 20080269173
Title: Composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin c derivatives
Abstract: Disclosed is a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives
The present invention relates to a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives.
BACKGROUND ART Generally, the mammalian hair follicle is a very organized, multi-layered and dynamic organ. The hair follicle includes epithelial cells such as inner and outer root sheath cells, matrix and hair shaft derived from the epithelial tissue, as well as dermal papilla and dermal sheath cell derived from the mesenchymal tissue. Interaction between the epithelial tissue and the mesenchymal tissue is necessary for hair growth after the birth, as well as development of the hair follicle.
The hair growth after the birth has a cell cycle of anagen, catagen and telogen. It has been known that the dermal papilla is encapsulated by epithelial matrix cell during the growth period, and growth factors, secreted in the dermal papilla such as insulin-like growth factor 1 (IGF-1), keratinocyte growth factor (KGF), etc., are differentiated to proliferate the epithelial cells and produce the hair shafts (Itami S, et. al., Biochem Biophys Res Commun. 212:988-94, 1995; Werner S, et. al., Science. 266:819-22, 1994).
Meanwhile, it was found that the epithelial cells undergoes programmed cell death (apoptosis) by the growth-inhibiting factors secreted in the dermal papilla such as tumor growth factor ß (TGF-ß) during the catagen (Soma et al., J Invest Dermatol. 111:948-54, 1998; Hibino and Nishiyama, J Dermatol Sci. 35:9-18, 2004). Accordingly, it has been known that the factors secreted in the dermal papilla cells control hair growth. Also, the size of the dermal papilla is in proportion to that of the hair follicle (Stenn and Paus, Physiol Rev. 81:449-494, 2001). Accordingly, it is expected that natural extracts or chemicals, which can control growth of the dermal papilla cells or their gene expression, play an important role in controlling the hair growth.
Vitamin C (L-ascorbic acid) exhibits a cofactor activity in the collagen synthesis, and is used as a supplementary component in the media. However, such a use of vitamin C is limited since the vitamin C is rapidly oxidized and degraded in an aqueous solution, especially under the general culture conditions. The vitamin C derivative L-ascorbic acid-2-phosphate magnesium (so-called, referred to as Asc 2-P) is a formulation that is more stable than the vitamin C, and has been used as a supplementary component in the various media. Like the vitamin C, Asc 2-P also stimulates growth of human dermal fibroblast and osteoblast (Hata et al., Eur J Biochem. 173:261-267, 1988). The vitamin C or the Asc 2-P has not been known at all to have what effect on the growth of hair and dermal papilla cells.
DISCLOSURE OF INVENTION Accordingly, the present invention is designed to solve the problems of the prior art, and therefore it is an object of the present invention to provide an agent for treating alopecia capable of stimulating growth of dermal papilla cells and/or hair follicles.
In order to accomplish the above object, the present invention provides a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives as an effective component.
In the present invention, the vitamin C derivatives are preferably selected from the group consisting of L-ascorbic acid-2-phosphate magnesium, L-ascorbyl palmitate and L-ascorbyl stearate, and L-ascorbic acid-2-phosphate magnesium of the following Chemistry FIG. 1 is the most preferred:
Also, the vitamin C derivatives of the present invention is characterized in that they promote growth of the hair follicles; stimulate growth of dermal papilla cells; stimulate production of an insulin like growth factor 1 (IGF-1) in the dermal papilla cells; increase expression of versican in the dermal papilla cells and the hair follicles; and increase expression of alkaline phosphatase in the dermal papilla cells.
http://www.freshpatents.com/Composition ... 269173.php
USPTO Application #: 20080269173
Title: Composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin c derivatives
Abstract: Disclosed is a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives
The present invention relates to a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives.
BACKGROUND ART Generally, the mammalian hair follicle is a very organized, multi-layered and dynamic organ. The hair follicle includes epithelial cells such as inner and outer root sheath cells, matrix and hair shaft derived from the epithelial tissue, as well as dermal papilla and dermal sheath cell derived from the mesenchymal tissue. Interaction between the epithelial tissue and the mesenchymal tissue is necessary for hair growth after the birth, as well as development of the hair follicle.
The hair growth after the birth has a cell cycle of anagen, catagen and telogen. It has been known that the dermal papilla is encapsulated by epithelial matrix cell during the growth period, and growth factors, secreted in the dermal papilla such as insulin-like growth factor 1 (IGF-1), keratinocyte growth factor (KGF), etc., are differentiated to proliferate the epithelial cells and produce the hair shafts (Itami S, et. al., Biochem Biophys Res Commun. 212:988-94, 1995; Werner S, et. al., Science. 266:819-22, 1994).
Meanwhile, it was found that the epithelial cells undergoes programmed cell death (apoptosis) by the growth-inhibiting factors secreted in the dermal papilla such as tumor growth factor ß (TGF-ß) during the catagen (Soma et al., J Invest Dermatol. 111:948-54, 1998; Hibino and Nishiyama, J Dermatol Sci. 35:9-18, 2004). Accordingly, it has been known that the factors secreted in the dermal papilla cells control hair growth. Also, the size of the dermal papilla is in proportion to that of the hair follicle (Stenn and Paus, Physiol Rev. 81:449-494, 2001). Accordingly, it is expected that natural extracts or chemicals, which can control growth of the dermal papilla cells or their gene expression, play an important role in controlling the hair growth.
Vitamin C (L-ascorbic acid) exhibits a cofactor activity in the collagen synthesis, and is used as a supplementary component in the media. However, such a use of vitamin C is limited since the vitamin C is rapidly oxidized and degraded in an aqueous solution, especially under the general culture conditions. The vitamin C derivative L-ascorbic acid-2-phosphate magnesium (so-called, referred to as Asc 2-P) is a formulation that is more stable than the vitamin C, and has been used as a supplementary component in the various media. Like the vitamin C, Asc 2-P also stimulates growth of human dermal fibroblast and osteoblast (Hata et al., Eur J Biochem. 173:261-267, 1988). The vitamin C or the Asc 2-P has not been known at all to have what effect on the growth of hair and dermal papilla cells.
DISCLOSURE OF INVENTION Accordingly, the present invention is designed to solve the problems of the prior art, and therefore it is an object of the present invention to provide an agent for treating alopecia capable of stimulating growth of dermal papilla cells and/or hair follicles.
In order to accomplish the above object, the present invention provides a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives as an effective component.
In the present invention, the vitamin C derivatives are preferably selected from the group consisting of L-ascorbic acid-2-phosphate magnesium, L-ascorbyl palmitate and L-ascorbyl stearate, and L-ascorbic acid-2-phosphate magnesium of the following Chemistry FIG. 1 is the most preferred:
Also, the vitamin C derivatives of the present invention is characterized in that they promote growth of the hair follicles; stimulate growth of dermal papilla cells; stimulate production of an insulin like growth factor 1 (IGF-1) in the dermal papilla cells; increase expression of versican in the dermal papilla cells and the hair follicles; and increase expression of alkaline phosphatase in the dermal papilla cells.
http://www.freshpatents.com/Composition ... 269173.php