Vitamin C derivatives in the stimulation of DP cells

[chore boy]

Composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin c derivatives
USPTO Application #: 20080269173

Title: Composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin c derivatives

Abstract: Disclosed is a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives

The present invention relates to a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives.

BACKGROUND ART Generally, the mammalian hair follicle is a very organized, multi-layered and dynamic organ. The hair follicle includes epithelial cells such as inner and outer root sheath cells, matrix and hair shaft derived from the epithelial tissue, as well as dermal papilla and dermal sheath cell derived from the mesenchymal tissue. Interaction between the epithelial tissue and the mesenchymal tissue is necessary for hair growth after the birth, as well as development of the hair follicle.

The hair growth after the birth has a cell cycle of anagen, catagen and telogen. It has been known that the dermal papilla is encapsulated by epithelial matrix cell during the growth period, and growth factors, secreted in the dermal papilla such as insulin-like growth factor 1 (IGF-1), keratinocyte growth factor (KGF), etc., are differentiated to proliferate the epithelial cells and produce the hair shafts (Itami S, et. al., Biochem Biophys Res Commun. 212:988-94, 1995; Werner S, et. al., Science. 266:819-22, 1994).

Meanwhile, it was found that the epithelial cells undergoes programmed cell death (apoptosis) by the growth-inhibiting factors secreted in the dermal papilla such as tumor growth factor ß (TGF-ß) during the catagen (Soma et al., J Invest Dermatol. 111:948-54, 1998; Hibino and Nishiyama, J Dermatol Sci. 35:9-18, 2004). Accordingly, it has been known that the factors secreted in the dermal papilla cells control hair growth. Also, the size of the dermal papilla is in proportion to that of the hair follicle (Stenn and Paus, Physiol Rev. 81:449-494, 2001). Accordingly, it is expected that natural extracts or chemicals, which can control growth of the dermal papilla cells or their gene expression, play an important role in controlling the hair growth.

Vitamin C (L-ascorbic acid) exhibits a cofactor activity in the collagen synthesis, and is used as a supplementary component in the media. However, such a use of vitamin C is limited since the vitamin C is rapidly oxidized and degraded in an aqueous solution, especially under the general culture conditions. The vitamin C derivative L-ascorbic acid-2-phosphate magnesium (so-called, referred to as Asc 2-P) is a formulation that is more stable than the vitamin C, and has been used as a supplementary component in the various media. Like the vitamin C, Asc 2-P also stimulates growth of human dermal fibroblast and osteoblast (Hata et al., Eur J Biochem. 173:261-267, 1988). The vitamin C or the Asc 2-P has not been known at all to have what effect on the growth of hair and dermal papilla cells.

DISCLOSURE OF INVENTION Accordingly, the present invention is designed to solve the problems of the prior art, and therefore it is an object of the present invention to provide an agent for treating alopecia capable of stimulating growth of dermal papilla cells and/or hair follicles.

In order to accomplish the above object, the present invention provides a composition for stimulating growth of dermal papilla cells and promoting hair follicle growth comprising vitamin C derivatives as an effective component.

In the present invention, the vitamin C derivatives are preferably selected from the group consisting of L-ascorbic acid-2-phosphate magnesium, L-ascorbyl palmitate and L-ascorbyl stearate, and L-ascorbic acid-2-phosphate magnesium of the following Chemistry FIG. 1 is the most preferred:

Also, the vitamin C derivatives of the present invention is characterized in that they promote growth of the hair follicles; stimulate growth of dermal papilla cells; stimulate production of an insulin like growth factor 1 (IGF-1) in the dermal papilla cells; increase expression of versican in the dermal papilla cells and the hair follicles; and increase expression of alkaline phosphatase in the dermal papilla cells.

http://www.freshpatents.com/Composition ... 269173.php

 

[chore boy]

Possible vendor:

http://www.peakchem.com/products/L-Asco ... nesium.htm

 

[chore boy]

. Induction of versican by ascorbic acid 2-phosphate in dermal papilla cells. Moon-Kyu Kim, Soon-Re Kim, Jung-Chul Kim, Young-Kwan Sung, Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea

We have recently reported that L-ascorbic acid 2-phosphate magnesium salt (Asc 2-P), a derivative of L-ascorbic acid (Vitamin C), promotes the elongation of hair shafts in hair follicles in culture and induces early conversion from a telogen phase to an anagen phase in mice, demonstrating that Asc 2-P affects hair growth and cycling. Here, we investigated whether Asc 2-P regulates expression of versican, which is thought to play an important role in anagen induction and maintenance of the normal hair growth, in cultured dermal papilla cells and in dermal papilla of isolated hair follicles in culture. Hair biopsy specimens were obtained from the non-balding occipital scalp region of patients with androgenic alopecia. Dermal papilla cells of the 2~3 passage were used in this study. Immunohistochemical staining of versican was performed with rabbit polyclonal antibody recognizing versican V0 and V1 isoforms. We observed that isoforms of versican were up-regulated in dermal papilla cells by Asc 2-P treatment. Immunohistochemical staining also revealed increased expression of versican, at least V0 and V1, in dermal papilla of isolated hair follicles treated with Asc 2-P. LY294002, a pharmacological inhibitor of PI3K, significantly attenuated Asc 2-P-induced versican expression. We also observed that Asc 2-P activates/phosphorylates PKB, a downstream effector of PI3K, and that LY294002 attenuates Asc 2-P-mediated phosphorylation of PKB. In addition, we observed that nuclear beta-catenin is increased by Asc 2-P in a dose-dependent manner and LY294002 reduces nuclear accumulation of beta-catenin. This study demonstrates that Asc 2-P induces versican expression via PI3K signaling and causes nuclear beta-catenin accumulation in dermal papilla cells. Since versican is thought to play an important role in anagen induction and maintenance of the normal hair growth, it would be worthy to evaluate vitamin C or its derivatives as a novel approach for treatment and prevention of hair loss.

http://www.ehrs.org/conferenceabstracts ... 03-kim.htm

 

[squeegee]

Nice find Chore but please get rid of that MJ pedo picture lol! This is horrible!

 

[Old Baldy]

You can get MAP at Skin Actives.com.

IMHO, the best way to use vitamin C is to just get the real thing. Put a small amount of ascorbic acid crystals into the palm of your hand, pour a little water into those crystals and dissolve as best you can and "slap" that wet concoction on your scalp. Put a little more water in your palm and slap that on to dissolve the crystals more if necessary.

Way cheaper and, remember, those stable vitamin C products are only trying to mimic the real thing.

If you were going to make a concoction and keep it for a while, then MAP would be great but using regular vitamin C, immediately after it is dissolved, is as good as it gets IMHO. (Just my opinion but, from all I've read, regular ascorbic acid is what the "stable" derivatives are trying to imitate.)

Tidbit: Don't use vitamin C with copper peptides. Use them many hours apart.

 

[chrome]

squeegee

brilliant avatar !

where did u get it...is there a website with similar avatars ?

cheers, chrome