- Reaction score
- 29
Hey guys.
I am a Neuropharmacology student from a prestigious Canadian university and I have recently been working on my honours thesis. Since it is not published yet I am unable to show the results of my research, but it is very promising.
I wanted to formulate a topical solution that was capable of growing hair on gene knockout mice who were otherwise incapable of growing hair on their own.
Using a combination of growth factors (which I was able to personally extract from human placental samples), PGE2 agonist, and a PGF2a agonist; my supervisor and I were able to stimulate endogenous hair growth.
With the results my research provided, and the addition of a few other chemicals to aid in HUMAN (not mice) hair growth (and defence against Androgenetic Alopecia) I was able to formulate a topical serum to be used on a human subject.
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The most notable difference was the addition of Superoxide Dismutase, an enzyme that is responsible for breaking down reactive oxygen species (ROS). Reactive oxygen species are defined as free radicals, which have the ability to damage cells and mutate genes. ROS have been implicated in hair loss (and even Androgenetic Alopecia) in a number of studies (E.g., http://www.ncbi.nlm.nih.gov/pubmed/19318119; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929555; http://www.ncbi.nlm.nih.gov/pubmed/23637859; http://www.nature.com/nm/journal/v12/n4/abs/nm1388.html) and Super Oxide dismutase has the ability to catalyze the breakdown of these ROS into non-harmful metabolites.
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Blue--> solvents
Dark purple--> Growth factors (implicated in HF neogenesis)
Light purple--> ROS eliminator
Grey--> GHK-Cu
Purple/purple--> Prostaglandin pathway
Orange--> stem cell pathway
Red--> androgen receptor
------------------------
Composition
------------------------
Deionized water
Dimetyl Isosorbide
Propylene Glycol
KGF
EGF
Superoxide Dismutase
GHK-Cu
PDE2 full agonist
PGF2a full agonist
CRTH2 inhibitor
GSK3B inhibitor
WNT activator
Androgen receptor antagonist
----------------------------------
I was able to place another order from a pharmaceutical grade chemical supplier (Sigma; using my supervisor's credentials) for my own "personal" research.
---------------------------------
It may look like quite the concoction, but most of the chemicals are dosed in the microgram range so it really isn't very much in solution itself. I'm currently in the preliminary phase of experimentation, just making sure my research subject will not grow horns or any other unwanted appendages from the application sites. The solution is quite stable, but just for precaution I keep it refrigerated. Application will begin with a total of [~1ml] applied to designated application site(s) daily for a minimum of 3 months before any deductions regarding the serums efficacy can be made. The research subject is currently approaching a NW2, with NW5 pattern similar to other members of his/her family.
Blood tests will be conducted once a week for the first month, and then biweekly thereafter to ensure systemic absorption is minimal (mostly for the topical AR antagonist).
--------------------------------
The experiment will begin on Monday, January 4th 2016 with pictures posted weekly regarding progress.
I will report back if anything changes.
Happy Holidays.
P.s. Shoutout to SwissTemples for providing me the information necessary (Swisstemples.com) to complete this.
I am a Neuropharmacology student from a prestigious Canadian university and I have recently been working on my honours thesis. Since it is not published yet I am unable to show the results of my research, but it is very promising.
I wanted to formulate a topical solution that was capable of growing hair on gene knockout mice who were otherwise incapable of growing hair on their own.
Using a combination of growth factors (which I was able to personally extract from human placental samples), PGE2 agonist, and a PGF2a agonist; my supervisor and I were able to stimulate endogenous hair growth.
With the results my research provided, and the addition of a few other chemicals to aid in HUMAN (not mice) hair growth (and defence against Androgenetic Alopecia) I was able to formulate a topical serum to be used on a human subject.
------------------------------
The most notable difference was the addition of Superoxide Dismutase, an enzyme that is responsible for breaking down reactive oxygen species (ROS). Reactive oxygen species are defined as free radicals, which have the ability to damage cells and mutate genes. ROS have been implicated in hair loss (and even Androgenetic Alopecia) in a number of studies (E.g., http://www.ncbi.nlm.nih.gov/pubmed/19318119; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929555; http://www.ncbi.nlm.nih.gov/pubmed/23637859; http://www.nature.com/nm/journal/v12/n4/abs/nm1388.html) and Super Oxide dismutase has the ability to catalyze the breakdown of these ROS into non-harmful metabolites.
-----------------------------
Blue--> solvents
Dark purple--> Growth factors (implicated in HF neogenesis)
Light purple--> ROS eliminator
Grey--> GHK-Cu
Purple/purple--> Prostaglandin pathway
Orange--> stem cell pathway
Red--> androgen receptor
------------------------
Composition
------------------------
Deionized water
Dimetyl Isosorbide
Propylene Glycol
KGF
EGF
Superoxide Dismutase
GHK-Cu
PDE2 full agonist
PGF2a full agonist
CRTH2 inhibitor
GSK3B inhibitor
WNT activator
Androgen receptor antagonist
----------------------------------
I was able to place another order from a pharmaceutical grade chemical supplier (Sigma; using my supervisor's credentials) for my own "personal" research.
---------------------------------
It may look like quite the concoction, but most of the chemicals are dosed in the microgram range so it really isn't very much in solution itself. I'm currently in the preliminary phase of experimentation, just making sure my research subject will not grow horns or any other unwanted appendages from the application sites. The solution is quite stable, but just for precaution I keep it refrigerated. Application will begin with a total of [~1ml] applied to designated application site(s) daily for a minimum of 3 months before any deductions regarding the serums efficacy can be made. The research subject is currently approaching a NW2, with NW5 pattern similar to other members of his/her family.
Blood tests will be conducted once a week for the first month, and then biweekly thereafter to ensure systemic absorption is minimal (mostly for the topical AR antagonist).
--------------------------------
The experiment will begin on Monday, January 4th 2016 with pictures posted weekly regarding progress.
I will report back if anything changes.
Happy Holidays.
P.s. Shoutout to SwissTemples for providing me the information necessary (Swisstemples.com) to complete this.
!!
