I followed the same method as I posted earlier in this thread. Multiple sources recommend spinning it at 2000rpm for 10 minutes so this is what I did. While working in a lab in the past I don't remember ever spinning it twice. 10 minutes at 2000 and I put two of the lavender/blue vials (you need this specific kind for the anticoagulant) and they separated very nicely and I suctioned the yellow plasma into a container.
I showered earlier and washed my hair. Then I washed my face with with tea tree oil. I cleaned my dermarollers with alcohol. I then applied a thin layer of the PRP to my balding areas of the scalp and dermarolled it really good in multiple directions, then I immediately applied more PRP to the balding areas.It spread nicely.
I then applied a small layer to my face and dermarolled it, then again applied more PRP when I was done.
So far so good. I plan on doing the hair treatment every week, and the face treatment once a month. The only thing I'm unsure about is if I want get blood drawn every week, or if I should get a larger amount drawn and use it for the whole month. How to store this I am unsure and I'm not sure if it would lose potency.
There is a few difference in spinning once compared to twice.
Spin it once, you have the whole plasma with the platelets in it and fibrinogen. Platelet concentration is lower.
Spin it twice and just extract the platelets and use the bottom part of the supernatant you get a higher platelet concentration and some fibrinogen. At times i just throw away all the supernatant and just use sterile saline to suspend the platelets.
If you are dermarolling, you need more volume than if you are injecting to cover the entire area. However if you are dermarolling, you need to add calcium to activate the platelets whereas if you inject it, the collagen in the tissue will activate the platelets.
Personally if I am injecting, 2mls is plenty for my scalp so spin it twice makes the most sense because I don't need volume and I get a concentrated product.
If I am dermarolling i will still spin it twice because there is just too much redundant volume with the entire chunk of plasma.
In essence, if you spin it once this is what you get (PRFM), platelet rich fibrin matrix. The fibrinogen will get converted to fibrin which will form a matrix and trap the platelets in it and this matrix of platelets release growth factors at a lower concentration for 1 week (the authors argued that this is physiological compared to the conventional highly concentrated PRP which does the release all at 1 time)
This only applies if you inject it only.
http://www.ncbi.nlm.nih.gov/pubmed/24810134
However the study did intradermal injections instead of dermarolling. I looked at all the PRP studies, I think this is the only (1 spin) study, the rest of the PRP studies are basically 2 spin types.