Rationale:Androgenetic alopecia is not considered a life threatening disease but can have serious impacts on the patient's psychosocial life. Genetic, hormonal, and environmental factors are considered responsible for the presence of androgenetic alopecia. Recent literature reports have proved the presence of inflammation and also of oxidative stress at the level of dermal papilla cells of patients with androgenetic alopecia Objective:We have considered of interest to measure the oxidative stress parameters in the blood of patients with androgenetic alopecia Methods and results:27 patients with androgenetic alopecia and 25 age-matched controls were enrolled in the study. Trolox Equivalent Antioxidant Capacity (TEAC), malondialdehyde (MDA) and total thiols levels were measured on plasma samples. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, and also non protein thiols levels together with TEAC activity were determined on erythrocytes samples No statistically significant changes were observed for TEAC erythrocytes, non-protein thiols, GPx and CAT activities. Significantly decreased (p<0.01) SOD activity was found in patients with androgenetic alopecia. For plasma samples decreased TEAC activity (p<0.001), increased MDA levels (p<0.001) and no change in total thiols concentration were found in patients when compared with the controls. Discussions:Decreased total antioxidant activity and increased MDA levels found in plasma samples of patients with androgenetic alopecia are indicators of oxidative stress presence in these patients. Significantly decreased SOD activity but no change in catalase, glutathione peroxidase, non protein thiols level and total antioxidant activity in erythrocytes are elements which suggest the presence of a compensatory mechanism for SOD dysfunction in red blood cells of patients with androgenetic alopecia.
Assessment of Zinc and Copper Contents in the Hair and Serum and Also Superoxide Dismutase, Glutathion Peroxidase and Malondi Aldehyde in Serum in Androgenetic Alopecia and Alopecia Areata.
Alopecia areata (AA) is a recurrent nonscarring type of hair loss that can affect any hair-bearing area. Androgenetic alopecia (Androgenetic Alopecia) or male pattern hair loss is a very common condition that has a significant psychosocial impact for patients. There are claims that imbalance of trace elements may trigger the onset of alopecia. The aim of this research was to assess the level of zinc, copper contents in the hair and level of lipid peroxidation as super oxide dismutase, Glutathion peroxidase and malon di aldehyde in serum in androgenic and areata alpecia. The prospective study was conducted in department of Dermatology of Sina Hospital and Biochemistry department of Tabriz University of medical science. The study population consists of 27 patients with alopecia areata and 27 patients with androgenetic alopecia. 27 age and sex matched healthy control subjects were studied. The level of zinc, copper contents in the hair and serum and level of lipid peroxidation as super oxide dismutase (SOD), Glutathion peroxidase(GPX-Px) and malon di aldehyde (MDA) in serum in androgenetic, areata alpecia and healthy control subjects were measured in all samples. The collected data were statistically analyzed, using SPSS. The duration of hair loss varied between 1-240 month among patients with alopecia areata and 6-180 month among patients with androgenetic alopecia. The mean of hair zinc level in AA patients, Androgenetic Alopecia patients and controls were 98.33 μg/dl, 105.35 μg/dl and 129.52 μg/dl. The mean level of hair copper level in study and control groups were, 7.91, 7.25 and 10.34. The mean of serum level of SOD, MDA and GPX-Px in study and control groups were (1945.25, 1861.57 and 2296.77), (3.64, 3.49 and 1.62) and (129.11, 118.84 and 138.74). Zinc and copper contents of hair and serum were significantly lower among patients with alopecia areata and androgenetic alopecia compared to controls (P<0.05). The serum level of SOD, GPX-Px were significantly lower and level of MDA were higher among patients with alopecia areata and androgenetic alopecia compared to controls (P<0.05). These results suggest that low levels of zinc and copper of hair and serum and lipid peroxidation and alterations in the oxidantantioxidant enzymatic system (SOD, GPX-Px) with high level of MDA at serum may play a role in the pathogenesis of AA and Androgenetic Alopecia.