More androgen tests on hairs...........from Japan

[michael barry]

TGF Beta 1 and hair growth"




Nippon Ronen Igakkai Zasshi. 2004 Nov;41(6):598-600.

Pathomechanism of androgenetic alopecia and new treatment



* Itami S.

Department of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University.

Hair follicles are composed primarily of epithelial and dermal components that develop from embryonic ectoderm and mesoderm respectively. The hair growth cycle is coordinated with complex processes that are dependent on the interactions of epithelial and dermal components. Beard and frontal scalp dermal papilla cells (DPCs) show the characteristics of androgen target cells. These DPCs expressed androgen receptor and type II 5alpha-reductase mRNA. To understand the mode of androgen action in human hair follicles, we developed an in vitro co-culture system using DPCs and follicular keratinocytes. Androgen significantly stimulated the proliferation of keratinocytes co-cultured with beard DPCs, suggesting that these DPCs produce androgen-dependent diffusible growth factors. Insulin-like growth factor-I (IGF-I) was identified as one of the androgen dependent paracrine growth factors from beard DPCs. On the other hand, we identified inhibitory roles of androgen on the growth of keratinocytes co-cultured with DPCs from human balding frontal scalp, when DPCs were transfected with the AR expression vector. This inhibitory effect was mediated by TGF-beta1 from the DPCs. Minoxidil and Finasteride were recently introduced for the treatment of androgenetic alopecia in Japan, and TGF-beta1 is the next target for innovative treatment.

 

[docj077]

Good find. I've read this study before.

A few people on here are trying to find a possible herb that could block the effects of TGF-beta. I think curcumin is the only thing we've found.

 

[michael barry]

barley proanthocyanidins can also I think.............let me look for the link http://www.ncbi.nlm.nih.gov/entrez/quer ... query_hl=7

 

[CCS]

procyanidin B-3

is barly the only source? Can we get it at a reasonable price? I got apple poly, 60 500mg capsules for $40. I plan to put one or two capsules in my minoxidil. I think that is just procyanidin B-2 but I'm not sure.

maybe i should start eating barly flour until then. I doubt it will do much. even if it does, it might grow body hair. so maybe I'll skip it.

 

[michael barry]

Proanthocyandin B-3 was what was tested College Chemistry Student. I dont know if B-2 has the same effect on TGF-beta one. I know that B-2 excited certain parts of the follicle.


Equally interesting is anthocyandins from blueberries. They are known to be very effective against prostate cancer. Would love to see them tested topically for anti-androgenic properties. I would imagine that they are also very potent anti-inflammatories also


If one wanted to stop TGF-beta one after transcription, B-3 proanthocyanidins is the only way I know of at this point. Other than, of course, blocking the androgen receptor and not having androgen transcribed beforehand

 

[CCS]

do you know where to get the blueberry ones or the B-3? I'll look for it and post what i can find.

 

[CCS]

http://www.nal.usda.gov/fnic/foodcomp/D ... yanidin%22

unsweatened backers chocolate and cocao are very high in Proanthocyandin's. Blueberries, granny smith apples, and red delicious apples are decently high.

cinnimon is extremely high, at 100g, though we won't use that much.

I wish i knew which ones are in cinnimon. We could use it as a topical, if we know it would not burn out scalps.

 

[CCS]

i just realized i don't have to put everything in a few bottles to avoid having to apply many topicals each day.

I just want to only apply topicals 2 times a day. At most 3 times. And preferably nothing very greasy before sociallizing.

Anyway, I'll put all my anti-oxidants in on bottle, and my minoxidil in the other, and my bosiwillia in another bottle, and my oils and fatty acids in all three. Then I can apply them one after the other in 5 minutes twice a day. I doubt they will react on my scalp. They might in a bottle if all together, but after application.

Anyway, I think my SOD, anti-oxidant mix is much cheaper than folligen or tricomin, so I will just use up my AC and folligen on my whole head for now and replace them with my mix soon enough. I'll start the mix now though.

I just wish i knew what kind of Proanthocyandin's are in grapeseed extract. I'm still searching for B-1 and B-3.

I'll keep the total concentration of all my anti-oxidants under 5%. Maybe 3-4%, in a representative mixture. This will be so cheap.

 

[CCS]

Carcinogenesis Advance Access originally published online on February 10, 2006
Carcinogenesis 2006 27(7):1445-1453; doi:10.1093/carcin/bgi347
This Article



PubMed

PubMed Citation
Articles by Veluri, R.
Articles by Agarwal, C.

© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Fractionation of grape seed extract and identification of gallic acid as one of the major active constituents causing growth inhibition and apoptotic death of DU145 human prostate carcinoma cells
Ravikanth Veluri 1, Rana P. Singh 1, Zhengjie Liu 1, John A. Thompson 1, 2, Rajesh Agarwal 1, 2 and Chapla Agarwal 1, 2, *
1 Department of Pharmaceutical Sciences, School of Pharmacy, 2 University of Colorado Cancer Center, University of Colorado Health Sciences Center, Denver, CO 80262, USA

* To whom correspondence should be addressed at: Department of Pharmaceutical Sciences, School of Pharmacy, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C238, Denver, CO 80262, USA. Tel: +1 303 315 1382; Fax: +1 303 315 6281; Email: Chapla.Agarwal@UCHSC.edu

The anti-cancer efficacy of grape seed extract (GSE) against prostate cancer (PCA) via its anti-proliferative, pro-apoptotic and anti-angiogenic activities in both cell culture and animal models have recently been described by us. GSE is a complex mixture containing gallic acid (GA), catechin (C), epicatechin (EC) and several oligomers (procyanidins) of C and/or EC, some of which are esterified to GA. To determine which components are most active against PCA, an ethyl acetate extract of GSE was separated by reverse-phase high-performance liquid chromatography (HPLC) into three fractions. Fraction 1 was far more effective than others in causing growth inhibition and apoptotic death of human PCA DU145 cells. Of the components in this fraction, GA showed a very strong dose- and time-dependent growth inhibition and apoptotic death of DU145 cells, but C and procyanidins B1 (EC–C dimer), B2 (EC–EC dimer) and B3 (C–C dimer) were nearly ineffective. Mechanistic studies demonstrated a strong caspase-9, caspase-3 and poly (ADP-ribose) polymerase (PARP) cleavages by GA in DU145 cells. Procyanidin oligomers eluting in HPLC Fractions 2 and 3 were obtained in larger quantities by separating GSE into eight fractions (I–VIII) on a gel filtration column. All fractions were analyzed by HPLC-UV and negative-ion electrospray mass spectrometry. Fractions I–III contained the active compound GA and inactive components C, EC, B1 and B2. Fraction IV contained other dimers and a dimer–GA ester and was also less active than GSE in DU145 cells. Fractions V–VIII, however, caused significant growth inhibition and apoptosis with the highest activity present in the later fractions that contained procyanidin trimers and GA esters of dimers and trimers. Together, these observations identify GA as one of the major active constituents in GSE. Several procyanidins, however, and especially the gallate esters of dimers and trimers also may be efficacious against PCA and merit further investigation.

 

[CCS]

http://www.ajcn.org/cgi/content/full/79/5/727

Proanthocyanidins differ from most other plant polyphenols because of their polymeric nature and high molecular weight. This particular feature should limit their absorption through the gut barrier, and oligomers larger than trimers are unlikely to be absorbed in the small intestine in their native forms. In vitro experiments using single layers of Caco-2 cells as a model of absorption in the small intestine showed that only the dimers and trimers of flavanols are able to cross the intestinal epithelium (114). Procyanidin B2 is very poorly absorbed in rats, whereas procyanidin B3 is not absorbed (115, 116). The possibility that procyanidin oligomers are hydrolyzed to mixtures of flavanol monomers and dimers in acidic conditions was suggested by Spencer et al from in vitro experiments (117). However, purified procyanidin dimer B3, as well as grapeseed proanthocyanidins having a higher degree of polymerization, are not degraded to more readily absorbable monomers in rats (116). The stability of proanthocyanidins was investigated in humans by regular analysis of gastric juice sampled with a gastric probe after ingestion of a proanthocyanidin-rich cocoa beverage (118). This study confirmed that proanthocyanidins are not degraded in the acidic conditions of the stomach in vivo. A minor absorption of some procyanidin dimers seems possible in humans. The procyanidin dimer B2 was detected in the plasma of volunteers after ingestion of a cocoa beverage; however, the maximal plasma concentration that was reached 2 h after ingestion was much lower than that reached after a roughly equivalent intake of epicatechin (0.04 compared with 6.0 µmol/L) (119). Proanthocyanidins, which are among the most abundant dietary polyphenols, are very poorly absorbed and may exert only local activity in the gastrointestinal tract or activity mediated by phenolic acids produced through microbial degradation. Their local action may nevertheless be important because the intestine is particularly exposed to oxidizing agents and may be affected by inflammation and numerous diseases such as cancer (120). Polyphenol concentrations in the colon can reach several hundred micromoles per liter (83), and together with a few carotenoids, polyphenols constitute the only dietary antioxidants present in the colon, because vitamins C and E are absorbed in the upper segments of the intestine.

 

[CCS]

very annoying. if only we could separate them somehow.

 

[CCS]

The article says our bodies can only absorb di-mers and tri-mers, not oligomers. Oligomers only works locally for intestinal health[, and maybe on the surface of the skin].

The USDA site states what food sources are high in the di-mers and tr-mers. The renette apples are very high, folled by red delicious, and then gala. The renette apples are the only ones without 4-mers etc. The other two have a lot of oligomers. I hope the apple poly I bought is not mostly oligomers.

baker's chocolate has a lot of all of them, so does cocoa, though mostly the polymers, beans are mostly polymers, and have a lot less after being boiled.

Yea! grape seeds and skins are high in dimers!

pecans and pistacios are almost as high as apples. This is all per 100g just so you remember.

white peaches are as high in dimers as the renette apples. yellow peaches are like the grany smith apples.

cinnimon has a lot, but only per 100g. curry powder has some too.

yellow plums and black plums are a bit higher.



the usda data base is very nice if you can find it and find the search feature.

 

[michael barry]

Collegechemistrystudent,

It may sound ridiculous, but I suppose one could just drink a couple of beers a day and eat some blueberries every day. Perhaps buy a blender and put it evey proanthocyandin-containing fruit a few times a week and make some sort of super anti-oxidant fruit punch and drink it with a couple of brews?


By the way......................Ive found out that Spectral DNC now has copper peptides nanosomally along with minxo aminexil and various herbs.


Ive also noticed Revlon now makes a hair-thinning shampoo, post shampoo liquid and some sort of serum. I would be very interested in the ingredients of what their well-financed labs have come up with.


Back to the pronanthocyandins.....................................youre a chemical guy.................would there be anyway to just put beer, various fruit extracts from blueberries, green apple peels, grape seeds, loniten tablets (minoxidil), barley and hops extract, and perhaps some beta-sitosterol tablets and some borage seed oil in a blender................................................................buzz it up. Then heat it on the stove...................and voila'=a new homemade topical with some alchohol to get it through the dermis? I wish there was more we could make at home (save money). I really cant believe that big cosmetics companies dont put out topical products with things that are effective for baldness on grocery store shelves other than just ol' minoxidil.


Wouldnt mind getting some roxythromicyn tablets into the above mentioned supertopical.

 

[CCS]

I don't know. I think my oleic acid, PPG, borageseed oili, water, ethanol, and octyl salicilate will last me a long time and do better than the beer. And the big bottles will last a long time. Beer will stink after a few hours. And buying extracts is very cheap. Just get the capsules, open one or two of each, and dump the contents in. Shake them up, do 3 months in advance so they have more time, and use the first one right away. If you don't want it greesy, use less PPG and oleic acid and OS.

 

[templemonk]

TGIF?