Home-made Revivogen

[michael barry]

Bryan wrote:

"Yeah, maybe, although I think it's fairly likely that the human scalp simply doesn't absorb these substances (RU, fatty acids, and especially EGCG) as well as rodent skin does, so the results are unlikely to be as dramatic as what you might expect from using the animal experiments as a guide."


I actually seen a study online about this (hamster flank organ permeability) and they found just that. I believe If I remember correctly it was on the order of four times or so easier to penetrate a flank organ's skin as human skin............................so it caused the researchers some concern as to whether or not testing stuff on a flank organ would necessarily translate to success in humans if a good enough vehichle for whatever topical wasn't found.

 

[abcdefg]

bryan your a topical kind of guy i remember you saying. Im curious what do you think are the most promising topicals out now and why? im just curious about what options in terms of topicals do we really have? I tried revivogen but I have a lot of hair so topicals arent really practical for me to apply.

 

[CCS]

abcdefg, that is why I make spironolactone shampoo, or put apple poly in my shampoo. I don't know if it will work, but it is better than nothing. Hey, piroctame olamine works. And your hair would not be an issue if you make homemade fatty acids. They are so cheap you could put more on your scalp. I bet it is the zink burning people's scalp that gives them the revivogen stains. It is not the fatty acids.

Micheal berry, I read it absorbs stuff 7x as well, put that also takes into account the fact that mice have a lot more hair follicles for the stuff to enter through. We don't care how much is absorbed, but rather how much gets to our follicle. So the only advantage the mice have on us is they have thinner skin. But we have bigger pores.

Bryan, I doubt the purity of EGCG is important. And I'm very convinced free fatty acids are absorbed well. Remeber reading about RUM? RU bonded to a free fatty acid? RU worked just fine on stump tail monkeys. So it is not just hampsters. Apple poly worked on humans. I agree we may not get as good of an effect, but we still can get some benifit. I'm not quiting. I just need to make this homemade revivogen work.

 

[CCS]

I know how to make homemade free fatty acids now

In my summer chemistry class, we have to hydrolyze the ester bonds in sucrose to get glucose and fructose. It says what I have to do to do this. Very easy, and most importantly, lab manual backed up.

This is good because it should work the same for breaking the bonds of oils to make free fatty acids. Best of all, I don't need much hydrochloric acid/base, since these are catalysts, and not bases.

Directions:
1. Use 1 drop of concentrated HCl (muriatic acid for cleaning pools. Sucks if you need a whole gallon) per milliliter of solution. Solution is oil on top of water.

2. Bring water to just under boiling for 5 minutes.

3. Bring back to neutral pH with NaOH (lye). You have to mix it with water, then add drops. The exact amounts I'd have to write out with my chemistry knowledge, but you people can sort of figure out if you buy some pH paper.

The glycerol should be sitting on the bottom, and the fatty acids floating on the top, unless the deprotenated acids are two soluble in water. Maybe separate the glycerol from the solution before going all the way back to pH 7. Or stop at pH 5, which is good enough.

Modificiations to procedure:
Sucrose is soluble in water, so it has high surface area with the HCl. Unfortunately, the oil does not disolve in water, so the reaction surface area is much less. Some people mix in alcohol to increase solubility, but I don't want to. I'll use a very active stir plate bar to mix it well. And I'll cook it for an hour instead of 5 minutes to make up for the reduced surface area.

I'll use 30mL water, about 30mL oil, and at least 5mL of 2M HCL in a 100mL flask, a jumping stir bar or tornato, heat to boiling, and let go for 1 hour, cool, separate, then remove glycerol and neutralize. Before I do this, I'll read up on what happens if HCl is added to C=C double bond. I don't want to break it and halogenate it. I hope a catalyst is needed for that, since the oils I want are highly non-saturated.


Oils: borageseed, and some flax and safflower. I'll do 3 flasks at once and make 75mL per day and mail it off to my brother.

 

[CCS]

If this works I can make 8oz for $6. I'll mail some for free to Bryan, Micheal Berry, Old Baldy, and Hairy for helping me out with stuff. I still have Bryan's mailing address. Maybe also HTGT. I can't go into business because I'm sure there is a patent.

 

[CCS]

I just want to point out that the borageseed oil is the most valuable when separated. Linoleic acid and Linolenic acid can be bought for $50 for 500mL at cityofchemicals.com, or some name like that. I'm making it way cheaper than that, but that pretty much puts a cap on what I could sell something for, except I don't have a $100 minimum. I certainly can't set up a web site, though. That would be sueable patent infrindgement.

 

[Bryan]

How do you plan on storing the fatty acids?

 

[CCS]

In plastic bottles in the fridge or freezer with maybe a little BHT in them. The bottle of BHT I have saysnot to use more than 0.02%, so that is a few small specs almost. I could put some CoQ10 powder in there too for good measure. The water remaining will even dissolve a little apple poly.

 

[CCS]

Now I know why my last fatty acid method did not seem to work. At a higher pH, the fatty acids are deprotonated and hold a lot of water. That was the gew I made. I need to be below pH 4 to get most of the fatty acids separated. Then they will be on top. So I did it right before, but I just did not know what I was looking at.

 

[CCS]

I just looked in my organic chemistry book. HCl does react with double bonds. No catalyst is needed, except for heat. I know that hot water would do the job too. But I still worry about the double bonds getting broken. I wonder how stable they are. It has to be possible, though.

 

[CCS]

I'm dumb. I just realized that sugars are bonded by ether bonds, not ester bonds. An ester bond has a double bonded oxygen.

Breaking the ester bond and not adding a Cl to the double bond depends on the activation energy for the double bond being higher, which means I better not use extreme measures that can break both. So Maybe only 80 degree C for longer time, and not as extreme of a pH.

Back not NaOH or HCl first. I think the electrophilic O=C, and the goal of protonating the carboxylic acid, points to HCl as my first choice. However, NaOH would dissolve the fatty acids into the water, and speed mixing, whereas HCl would keep them protonated and mixed with the oil, slowing the reaction. Also, NaOH would risk a glycol biproduct, whereas HCl risks a halogenated biproduct. I also know that NaOH does work, since it works with soaps. I'd just like to find the activation energies.

I wonder if OH- breaks double bonds. If not, it would be a good choice.

 

[CCS]

a little hunting around is telling me that acids don't need catalysts, and have little trouble protonating a double bond. All the NaOH attacks are accompanied by catalysts, though.

 

[CCS]

http://www.vanderbilt.edu/AnS/Chemistry ... slides.pdf

Bases can remove the halogenation, though.

 

[CCS]

I think I'll try 8 drops of 2M NaOH in 70mL of liquid (40mL oil) in a 100mL Erlenmeyer flask, and stir vigerouly and heat to 90 degrees C for 1 hour, then let cool.

I just read that glycerol is water soluble. That complicates things. I'll need a pH of 4 or so to separate the acids from the water and glycerol.

Check out the benifits of linoleic acid on the skin:
http://en.wikipedia.org/wiki/Linoleic_a ... trial_uses

I'm not just going to put this stuff on my head. I'm putting it on my face and neck.

 

[Jacob]

Elsom Research- The Formulator.

It's a lot easier for me anyway :lol:

 

[CCS]

I just realized the newly formed acids will get rid of the OH- catalysts. But if I use an H+ catalyst, it can damage the double bonds. Going the OH route, I need a lot of Lye. About 10% the mass of the oil, and that would not go unnoticed. Maybe the alcohol and straight heat idea is better. Or PG, since it won't evaporate and I have a ton.

Need more google searching.

yeah, sugars don't have double bonds, so HCl is great for them.

 

[Jacob]

Why not have Elsom whip something up? Nanosomal EGCG..etc etc.

 

[CCS]

xx

 

[CCS]

Look how complicated this company does it. And I was going to try doing it myself.


http://www.freepatentsonline.com/4100181.html

United States Patent 4100181 Link to this page:http://www.freepatentsonline.com/4100181.html Abstract:Substantial benefits and economies are realized in a process for obtaining free fatty acids from soap stock by first acidulating the soap stock to liberate fatty acids from the soap present, separating the resulting first acid oil and aqueous saline phases, recovering free fatty acids by vacuum distillation from this first acid oil phase, saponifyng the glyceride-rich still residue to generate additional soap, acidulating the saponified product to liberate additional fatty acids therefrom and separating the resulting second acid oil and aqueous saline phases. These additional fatty acids can be recovered as free acids by vacuum distillation from the separated second acid oil phase. It is sometimes advantageous to neutralize residual mineral acid in the first acid oil phase before distillation.

 

[Jacob]

I didn't realize Revivogen was so expensive and hard to get :?