Interesting questions, I will get back to you on some later, but regarding how long the regenerated HF last, my reading of this is:
HF have been generated through some of the methods of DP/Epidermis/organoids but the effect did NOT last. Indeed, we have seen so many follicles on the back of mice, but they did not last long. Which is why Tsuki's latest paper is important, because that was his focus: cycling, and the environment needed for that.
HOWEVER: This is NOT a problem with wound-induced hairs.
In WIHN, a fully functional follicle can regenerate in the centre of a full-thickness wound with a large enough size, and the cellular origin of this process is similar to an embryonic process. The neogenic follicles have similar functions to embryonic HFs, which also have a growth cycle.
In other words: The elusive wound-healing method such as Follica has more support for potential long term growth as opposed to the other methods so far (instead of using DC cells/injecting them/and so on).
Having said that, Follica has not succeded yet at all. But that could be from lack of trying/luck. It may very well be, that those amazing success stories from wounding + minoxidil that they have generated de-novo hair follicles. We can never be sure without an analysis, but their results are impressive enough to suggest that this might be the case. What do you think? Is that the overall impression?
After reading bunch of papers in the last few weeks, I really think that WIHIN holds the key for an earlier treatment, as the other methods will take a long time to be figured out.
BTW a lot of questions remain to be solved:
How long can the regenerated HFs last? Can the regenerated HFs go through full hair cycling