Functional Characterization Of A Small-molecule Inhibitor Of The Dkk1-lrp6 Interaction

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https://www.hindawi.com/journals/isrn/2012/823875/

Figure 1: NCI8642 is able to abolish DKK1-LRP6 interaction at the cell surface. (A) Immunostaining of DKK1 on HEK293 cells (panel a), on HEK293-LRP6 cells (panel b), on HEK293-LRP6 cells in the presence of 1% DMSO (panel c), and on HEK293-LRP6 cells in the presence of 100 μM NCI8642 (panel d). Immunostaining of AP-DKK1 on HEK293-LRP6 cells (panel e and f). The cell-bound DKK1 was detected with anti-DKK1 antibody. (B) Enzymatic quantification of AP-DKK1 binding to HEK293-LRP6 cells, in the presence of increasing concentrations of NCI8642.
 
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