http://www.jbc.org/cgi/reprint/278/28/26120.pdf
The hair growth cycle consists of three stages known as the anagen (growing), catagen (involution), and telogen
(resting) phases. This cyclical growth of hair is regulated by a diversity of growth factors. Although normal
expression of both epidermal growth factor and its receptor (EGFR) in the outer root sheath is down-regulated
with the completion of follicular growth, here we show that continuous expression of epidermal growth
factor in hair follicles of transgenic mice arrested follicular development at the final stage of morphogenesis.
Data from immunoprecipitation and immunoblotting showed that epidermal growth factor signals through
EGFR/ErbB2 heterodimers in skin. Furthermore, topical application of tyrphostin AG1478 or AG825, specific
inhibitors of EGFR and ErbB2, respectively, completely inhibited new hair growth in wild type mice but not in
transgenic mice. When the transgenic mice were crossed with waved-2 mice, which possess a lower kinase activity
of EGFR, the hair phenotype was rescued in the offspring. Taken together, these data suggest that EGFR
signaling is indispensable for the initiation of hair growth. On the other hand, continuous expression of
epidermal growth factor prevents entry into the catagen phase. We propose that epidermal growth factor functions
as a biologic switch that is turned on and off in hair follicles at the beginning and end of the anagen
phase of the hair cycle, guarding the entry to and exit from the anagen phase.
People attempting Follica's method at home...well there you go, its not just about inhibiting EGF(R) - the inhibition needs to be timed!
The hair growth cycle consists of three stages known as the anagen (growing), catagen (involution), and telogen
(resting) phases. This cyclical growth of hair is regulated by a diversity of growth factors. Although normal
expression of both epidermal growth factor and its receptor (EGFR) in the outer root sheath is down-regulated
with the completion of follicular growth, here we show that continuous expression of epidermal growth
factor in hair follicles of transgenic mice arrested follicular development at the final stage of morphogenesis.
Data from immunoprecipitation and immunoblotting showed that epidermal growth factor signals through
EGFR/ErbB2 heterodimers in skin. Furthermore, topical application of tyrphostin AG1478 or AG825, specific
inhibitors of EGFR and ErbB2, respectively, completely inhibited new hair growth in wild type mice but not in
transgenic mice. When the transgenic mice were crossed with waved-2 mice, which possess a lower kinase activity
of EGFR, the hair phenotype was rescued in the offspring. Taken together, these data suggest that EGFR
signaling is indispensable for the initiation of hair growth. On the other hand, continuous expression of
epidermal growth factor prevents entry into the catagen phase. We propose that epidermal growth factor functions
as a biologic switch that is turned on and off in hair follicles at the beginning and end of the anagen
phase of the hair cycle, guarding the entry to and exit from the anagen phase.
People attempting Follica's method at home...well there you go, its not just about inhibiting EGF(R) - the inhibition needs to be timed!
