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Oxidative stress plays a direct role in mediating DHT-stimulated TGF-β secretion by dermal papilla cells
[h=1]Oxidative stress plays a direct role in mediating DHT-stimulated TGF-β secretion by dermal papilla cells[/h]Article notes ► Copyright and License information ►
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Jamie Upton[SUP]*[/SUP], Adiam Bahta, Nilofer Farjo[SUP]1[/SUP], Bessam Farjo[SUP]1[/SUP], Mike Philpott
Barts and The London School of Medicine and Dentistry, Queen Mary University of London, UK; [SUP]1[/SUP]Farjo Medical Centre, Manchester, UK. [SUP]*[/SUP]E-mail:
We investigated the effects of oxygen on secretion of TGF-β in response to dihydrotestosterone (DHT). Dermal papilla cells (DPCs) from frontal hair follicles were grown as explant cultures from biopsies donated by patients undergoing hair transplant. DPCs were split between normal tissue culture conditions of 5% CO[SUB]2[/SUB] 20% O[SUB]2[/SUB] or low oxygen conditions of 5% CO[SUB]2[/SUB] 2% O[SUB]2[/SUB]. We have previously shown that under 20% O[SUB]2,[/SUB] frontal DPCs exhibit reduced growth rate and higher levels of reactive oxygen species (ROS) compared with those grown under 2% O[SUB]2[/SUB]. We show that the ROS state of DPCs correlates with the effects of DHT on TGF-β secretion. We found that under 20% O[SUB]2[/SUB] DHT stimulated TGF-β secretion, as previously published (Inui et al. 2002), whereas 2% O[SUB]2[/SUB] negates and in some cases may even reverse DHT-stimulated TGF-β secretion by DPCs. Moreover, by using hydrogen peroxide to chemically induce oxidative stress, we observed an increase in the amounts of TGF-β secreted by the DPCs. These data suggest that oxidative stress may exacerbate the onset of androgenic alopecia by affecting TGF-β secretion, a known inhibitor of hair follicle growth and inducer of catagen.
[h=1]Oxidative stress plays a direct role in mediating DHT-stimulated TGF-β secretion by dermal papilla cells[/h]Article notes ► Copyright and License information ►
Review date: . Status:
Copyright notice
Jamie Upton[SUP]*[/SUP], Adiam Bahta, Nilofer Farjo[SUP]1[/SUP], Bessam Farjo[SUP]1[/SUP], Mike Philpott
Barts and The London School of Medicine and Dentistry, Queen Mary University of London, UK; [SUP]1[/SUP]Farjo Medical Centre, Manchester, UK. [SUP]*[/SUP]E-mail:
We investigated the effects of oxygen on secretion of TGF-β in response to dihydrotestosterone (DHT). Dermal papilla cells (DPCs) from frontal hair follicles were grown as explant cultures from biopsies donated by patients undergoing hair transplant. DPCs were split between normal tissue culture conditions of 5% CO[SUB]2[/SUB] 20% O[SUB]2[/SUB] or low oxygen conditions of 5% CO[SUB]2[/SUB] 2% O[SUB]2[/SUB]. We have previously shown that under 20% O[SUB]2,[/SUB] frontal DPCs exhibit reduced growth rate and higher levels of reactive oxygen species (ROS) compared with those grown under 2% O[SUB]2[/SUB]. We show that the ROS state of DPCs correlates with the effects of DHT on TGF-β secretion. We found that under 20% O[SUB]2[/SUB] DHT stimulated TGF-β secretion, as previously published (Inui et al. 2002), whereas 2% O[SUB]2[/SUB] negates and in some cases may even reverse DHT-stimulated TGF-β secretion by DPCs. Moreover, by using hydrogen peroxide to chemically induce oxidative stress, we observed an increase in the amounts of TGF-β secreted by the DPCs. These data suggest that oxidative stress may exacerbate the onset of androgenic alopecia by affecting TGF-β secretion, a known inhibitor of hair follicle growth and inducer of catagen.
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