michael barry
Senior Member
- Reaction score
- 12
S-12-B
Nanoparticle-based Targeting of Skin Antigen-
Presenting Cells via Hair Follicles
Speaker: Annika Vogt
Clinical Research Center for Hair and Skin Physiology,
Berlin, Germany
Authors: Annika Vogt1, Brice Mahe2, Wolfram Sterry1,
Behazine Combadiere2, Ulrike Blume-Peytavi1
1 Clinical Research Center for Hair and Skin Physiology,
Department of Dermatology and Allergy, Charite –
Universitaetsmedizin Berlin, Berlin, Germany;
2 Laboratoire d’Immunologie Cellulaire et Tissulaire,
Hôpital Pitié Salpêtrière, INSERM U543, Paris, France
Drug delivery systems, which target active compounds
to the hair follicle, may result in a better penetration and
a higher ef. ciency of hair and skin therapy. Recent studies
performed by our group suggest, that nanoparticles in
the size range of 40 nm may be used to transcutaneously
deliver active vaccine compounds, via the hair follicle, into
cutaneous antigen-speci. c cells. To further investigate
the applicability of transcutaneously applied nanoparticles
as vaccine carriers, we investigated the penetration and
the migratory pro. le of 40 nm nanoparticles through the
skin and to secondary lymphoïd organs of C57BL6 mice
using in vivo confocal microscopy. We found that 40 nm
nanoparticles penetrated deeply into open hair follicles
of tape-stripped murine skin. Within 24 hrs diffusion into
the perifollicular tissue occurred, and, concomitantly,
nanoparticle-positive cells could be identi. ed in proximal
draining lymph nodes, mesenteric lymph nodes and the
spleen. Transcutaneous application of immunogenic
compounds such as DNA plasmids encoding for ovalbumin
(OVA) or OVA itself induced proliferation of OVA-speci. c
CD8 T cells. Similarly, transcutaneously applied human
in. uenza vaccine elicited antigen-speci. c T cells assessed
by IFN ELISPOT. Our results further strengthen our concept
transcutaneous targeting of cutaneous antigen-presenting
cells. Further studies using functional particle-bound
antigens will help to validate this route of immunisation.
Nanoparticle-based Targeting of Skin Antigen-
Presenting Cells via Hair Follicles
Speaker: Annika Vogt
Clinical Research Center for Hair and Skin Physiology,
Berlin, Germany
Authors: Annika Vogt1, Brice Mahe2, Wolfram Sterry1,
Behazine Combadiere2, Ulrike Blume-Peytavi1
1 Clinical Research Center for Hair and Skin Physiology,
Department of Dermatology and Allergy, Charite –
Universitaetsmedizin Berlin, Berlin, Germany;
2 Laboratoire d’Immunologie Cellulaire et Tissulaire,
Hôpital Pitié Salpêtrière, INSERM U543, Paris, France
Drug delivery systems, which target active compounds
to the hair follicle, may result in a better penetration and
a higher ef. ciency of hair and skin therapy. Recent studies
performed by our group suggest, that nanoparticles in
the size range of 40 nm may be used to transcutaneously
deliver active vaccine compounds, via the hair follicle, into
cutaneous antigen-speci. c cells. To further investigate
the applicability of transcutaneously applied nanoparticles
as vaccine carriers, we investigated the penetration and
the migratory pro. le of 40 nm nanoparticles through the
skin and to secondary lymphoïd organs of C57BL6 mice
using in vivo confocal microscopy. We found that 40 nm
nanoparticles penetrated deeply into open hair follicles
of tape-stripped murine skin. Within 24 hrs diffusion into
the perifollicular tissue occurred, and, concomitantly,
nanoparticle-positive cells could be identi. ed in proximal
draining lymph nodes, mesenteric lymph nodes and the
spleen. Transcutaneous application of immunogenic
compounds such as DNA plasmids encoding for ovalbumin
(OVA) or OVA itself induced proliferation of OVA-speci. c
CD8 T cells. Similarly, transcutaneously applied human
in. uenza vaccine elicited antigen-speci. c T cells assessed
by IFN ELISPOT. Our results further strengthen our concept
transcutaneous targeting of cutaneous antigen-presenting
cells. Further studies using functional particle-bound
antigens will help to validate this route of immunisation.