The Human Hair Cycle and Epidermal Proteins

May 17 21:15 2016 Print This Article

Hair Loss Studies

Expression of the Proteins Associated with Epidermal Differentiation During Human Hair Growth Cycle


Yong-Jun Piao, Ki-Beom Suhr
Jeung-Hoon Lee, Jang-Kyu Park
Department of Dermatology, School of Medicine
Chungnam National University Daejeon, Korea.

Study Information and Results:

The profound morphological and molecular changes in the human hair follicles are involved in the transition from anagen to catagen or telogen.

In order to investigate how the expression of epidermal proteins associated with differentiation also occurs in hair follicles according to its cycle, we performed immunohistochemistry and western blotting, using anagen and catagen hair follicles.

In immunohistochemical staining, trichohyalin was expressed at inner root sheath (IRS) and medula in anagen hair follicle, but disappeared in catagen stage. Involucrin was expressed at IRS and innermost cell (IMC) of outer root sheath (ORS) in anagen hair follicle, and diffusely spreaded into wider area of ORS in catagen stage. Small protein rich proteins (SPR) was expressed in IMC layer in anagen and catagen stage.

Filaggrin, positive staining was observed upper portion of keratinization in IMC of anagen hair follicles, it was disappeared in catagen stage. Transglutaminase (TGase) 1 and 3 were expressed in the greater part of areas of anagen hair follicles.

Western blotting showed that the expressions of the proteins for epidermal differentiation (THH, involucrin, TGase1, TGase3, cytokeratin17) were observed in anagen and catagen hairs, similarly.

These data may be helpful to understand the relationship between human hair cycle and the expression of epidermal proteins associated with differentiation.