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Increased androgen receptor messenger RNA in frontal-parietal hair follicles of women with androgenetic alopecia.
Richeti F, Kochi C, Rocha MN, Sant'anna Corrêa C, Lazzarini R, Guazzelli RM, Mendonça RF, Melo MR, Longui CA.
Source
Laboratório de Medicina Molecular, Departamento de Ciências Fisiológicas, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP, Brasil.
Abstract
Normal testosterone levels are frequently observed in women with androgenetic alopecia (Androgenetic Alopecia), suggesting the involvement of androgen sensitivity in this condition. Androgen sensitivity is related to androgen receptor (AR) messenger RNA (mRNA) production in hair follicles and is negatively related to the number of CAG repeats present in exon 1 of the AR gene. The aim of this study was to compare AR expression in Androgenetic Alopecia women with normal controls and to correlate this expression with the number of CAG repeats. Hair follicles were obtained from 27 women with Androgenetic Alopecia and 21 controls for AR gene expression analysis. AR expression was evaluated through AR mRNA quantification using real-time polymerase chain reaction and the number of CAG repeats in the AR gene was determined in complementary DNA samples obtained from hair follicles and analyzed with the Gene Scan software. AR mRNA in the frontal-parietal region was significantly higher than that in the occipital region of Androgenetic Alopecia patients (paired t-test, P = 0.046). No significant difference was identified in controls (P = 0.67). Both regions in the same individual showed a significant positive correlation both in Androgenetic Alopecia patients (r = 0.77; P < 0.05) and in controls (r = 0.91; P < 0.05). A negative correlation was identified between AR expression and the number of CAG repeats only in Androgenetic Alopecia patients (r = 0.510, P = 0.013). The identification of elevated AR mRNA quantitation in hair follicles is a useful tool for identifying potentially abnormal androgen sensitivity in Androgenetic Alopecia patients.
Richeti F, Kochi C, Rocha MN, Sant'anna Corrêa C, Lazzarini R, Guazzelli RM, Mendonça RF, Melo MR, Longui CA.
Source
Laboratório de Medicina Molecular, Departamento de Ciências Fisiológicas, Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP, Brasil.
Abstract
Normal testosterone levels are frequently observed in women with androgenetic alopecia (Androgenetic Alopecia), suggesting the involvement of androgen sensitivity in this condition. Androgen sensitivity is related to androgen receptor (AR) messenger RNA (mRNA) production in hair follicles and is negatively related to the number of CAG repeats present in exon 1 of the AR gene. The aim of this study was to compare AR expression in Androgenetic Alopecia women with normal controls and to correlate this expression with the number of CAG repeats. Hair follicles were obtained from 27 women with Androgenetic Alopecia and 21 controls for AR gene expression analysis. AR expression was evaluated through AR mRNA quantification using real-time polymerase chain reaction and the number of CAG repeats in the AR gene was determined in complementary DNA samples obtained from hair follicles and analyzed with the Gene Scan software. AR mRNA in the frontal-parietal region was significantly higher than that in the occipital region of Androgenetic Alopecia patients (paired t-test, P = 0.046). No significant difference was identified in controls (P = 0.67). Both regions in the same individual showed a significant positive correlation both in Androgenetic Alopecia patients (r = 0.77; P < 0.05) and in controls (r = 0.91; P < 0.05). A negative correlation was identified between AR expression and the number of CAG repeats only in Androgenetic Alopecia patients (r = 0.510, P = 0.013). The identification of elevated AR mRNA quantitation in hair follicles is a useful tool for identifying potentially abnormal androgen sensitivity in Androgenetic Alopecia patients.