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Biol Pharm Bull. 2005 Mar;28(3):485-9.
A Short Peptide GPIGS Promotes Proliferation of Hair Bulb Keratinocytes
and Accelerates Hair Regrowth in Mice.
Tsuruda A, Kawano Y, Maekawa T, Oka S.
Institute for Biological Resources and Functions, National Institute of
Advanced Industrial Science and Technology (AIST), Japan.
The aim of this study was to discover a novel agent that promotes hair
growth. We carried out a screening test in 298 types of conditioned
medium (CM) from cultures of bacteria by using a hair bulb keratinocyte
(HBK) growth assay. As a result, we found a HBK growth factor in the CM
of Bacillus sp. M18. This HBK growth factor was purified by collecting
biologically active fractions in three steps, including HP-20 batch
processing, LH-20 chromatography and C(18) reverse-phase high-pressure
liquid chromatography, and identified as a short peptide GPIGS. GPIGS
increased Akt phosphorylation in HBKs. Moreover, the GPIGS-stimulated
HBK growth was inhibited by the treatment with LY294002, an inhibitor of
phosphatidylinositol 3-kinase (PI-3K). These results suggest that GPIGS
promotes HBK growth via the PI-3K/Akt pathway. In addition to in vitro
tests, GPIGS was found to accelerate hair regrowth in telogen mice. Our
results indicate that GPIGS is a potential agent to promote hair growth.
PMID: 15744074
A Short Peptide GPIGS Promotes Proliferation of Hair Bulb Keratinocytes
and Accelerates Hair Regrowth in Mice.
Tsuruda A, Kawano Y, Maekawa T, Oka S.
Institute for Biological Resources and Functions, National Institute of
Advanced Industrial Science and Technology (AIST), Japan.
The aim of this study was to discover a novel agent that promotes hair
growth. We carried out a screening test in 298 types of conditioned
medium (CM) from cultures of bacteria by using a hair bulb keratinocyte
(HBK) growth assay. As a result, we found a HBK growth factor in the CM
of Bacillus sp. M18. This HBK growth factor was purified by collecting
biologically active fractions in three steps, including HP-20 batch
processing, LH-20 chromatography and C(18) reverse-phase high-pressure
liquid chromatography, and identified as a short peptide GPIGS. GPIGS
increased Akt phosphorylation in HBKs. Moreover, the GPIGS-stimulated
HBK growth was inhibited by the treatment with LY294002, an inhibitor of
phosphatidylinositol 3-kinase (PI-3K). These results suggest that GPIGS
promotes HBK growth via the PI-3K/Akt pathway. In addition to in vitro
tests, GPIGS was found to accelerate hair regrowth in telogen mice. Our
results indicate that GPIGS is a potential agent to promote hair growth.
PMID: 15744074