The antiandrogen effect of the banjiro leaf extract (50% ethanol eluted fraction) was 44.5% at 1 ?g/mL. The cell viability was 110%. So, since the banjiro leaf extract was contained in up to 90% ethanol eluted fraction, the 50% ethanol eluted fraction has the antiandrogen effect.
Proliferative efficacy of hair follicle cells
Hair follicle cells (outer root sheath cells) are cells formed by differentiation of hair matrix cells at the upper dermal papilla. They become hair and grow by division, growth, and keratinisation. The growth of hair matrix cells and hair follicle cells decreases with the effects of the testosterone, stress, and nutrition disorders. If their growth decreases, the hair growth rate declines and hair becomes thinner, resulting in the development and progression of alopecia. The proliferative effect of the banjiro leaf extract on hair follicle cells was confirmed.
Hair follicle cells obtained from the back of a mouse were added to a medium containing sample solid compound 10 ?g/mL and cultured. After culture, the cell growth was measured by MTT assay.
The banjiro leaf extract promoted cell growth by 132.6% compared to the control (100%).
Proliferative efficacy of hair dermal papilla cells
The growth rate of dermal papilla cells, which constitute the dermal papilla, declines with the effect of the testosterone, and the size of the dermal papilla is decreased. This results in thinner hair and the development and progression of alopecia. The proliferative effect of the banjiro leaf extract on dermal papilla cells was confirmed.